Stroebech Heavy Oil 

50 ml Peroxide tested Pre-washed Oil

Description and Prod. No.

50 ml Oil for in glass bottle Prod. No. 2.09.050

Stroebech Oil is a pharmaceutical grade light mineral oil*), pre-washed and sterile filtered light paraffin oil. Oil is often the key to poor results, use only BEA tested and Peroxide Value (POV) tested oil as minimum standard quality control.

*) Liquid paraffin oil is a mineral oil and is a by-product of crude oil distillation.

Oil viscosity influences temperature, pH and osmolarity, with higher viscosity demonstrating higher yields after overlay in embryo culture in terms of blastocyst formation rates and cell counts.

Advantages: easier to handle, droplets are maintained better, and oil doesn’t spill over as easy.

Temperature, pH is better maintained and increased media osmolarity is avoided both during handling and during culture. This has a positive impact on blastocyst rate formation – especially for the longer culture periods required in some species.

The unit of viscosity is newton-second per square metre, which is usually expressed as pascal-second in SI units, cP.

We have increased the viscosity from < 30 cP to 95-140 cP.

Quality Control: we still apply the strict quality control tests of each batch which besides a bovine embryo assay (BEA) test also includes *) POV, sterility, and endotoxin tests.

*) The peroxide value (POV) is defined as the reactive oxygen contents expressed in terms of milliequivalents (meq) of free iodine per kilogramme of fat. It is determined by titrating iodine liberated from potassium iodide with sodium thiosulphate solution. Oils with POV well below 10 meq/kg are considered fresh – The limit for our oil is <0.1meq/kg

Quality Control Tests and Manufacturing

  • Sterility tested (Fungal and Bacterial)
  • Osmolality tested and pH tested
  • Endotoxin tested
  • Manufacturer is ISO9001 and ISO13485 certified
  • Controlled environment and to human standards
  • Bovine Embryo Assay (BEA)
  • Batch analysis certificate available

Storage:

  • Store in original unopened container refrigerated 3-9 °C and protected from light
  • Do not expose to temperatures higher than 39°C
  • Do not freeze product it may precipitate < 2°C
  • The medium must be used within 10 days after opening, providing it has been
  • handled aseptically and is kept refrigerated after opening
  • Discard unused medium if it has been warmed

Contact: www.stroebech-media.com - Phone: +45 5353 8743