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Every media batch is provided with a Certificate of Analysis with a high level of Quality Control (QC) release parameters.
The most important is the Bovine Embryo Assay BEA Test. There are huge differences in reproductive physiology between the mouse and cow, therefore a MEA test is not sufficient QC for a bovine medium.
See more about our QC and Manufacturing here
As a customer you get a detailed Manual of Procedure and access to online support. Furthermore, there will be online training courses and individual troubleshooting sessions.
All the media are serum-free and ready-to-use and require no supplements.
The media range applies to in vitro production of bovine embryos from both Ovum Pick Up and Slaughterhouse ovary collection. The media can also be used to produce sheep, goat, buffaloes and camelid embryos. For endangered species, ZOO- and wildlife animals
Contact us for custom made solutions.
500mlProd. No. 6.01.500
For retrieval of oocytes from Ovum Pick Up and for Embryo Flushing (ET)
Calcium Chloride, Glucose, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Heparin, Sodium Lactate, Sodium Pyruvate, HEPES and Gentamycin
50mlProd. No. 5.02.050
For handling of oocytes and embryos outside of the incubator
Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin
20mlProd. No. 5.03.020
For in vitro maturation of oocytes in the laboratory
Leptin, FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), L-Carnitine, Sodium Pyruvate, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
20mlProd. No. 5.04.020
For the in vitro maturation of oocytes outside the incubator
Leptin, HEPES, FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), L-Carnitine, Sodium Pyruvate, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
50mlProd. No. 5.05.050
For washing of semen during centrifugation prior to in vitro fertilization
Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin
20mlProd. No. 5.06.020
For in vitro fertilization of matured oocytes
Essential and non-essential amino acids, PHE (Penicillamine, Hypotaurine, Epinephrine), Heparin, Albumin, Sodium Pyruvate, Inorganic Salts, Essential and Non-Essential Amino Acids, Ca-L-Lactate, Sodium Bicarbonate, ALA-Glutamine, Glucose, Phenol Red, and Gentamycin
20mlProd. No. 5.07.020
For in vitro culture of embryos
Insulin, Hyaluronic Acid, EDTA, Calcium L-Lactate, Sodium Selenite, L-Carnitine, Ethanolamine, Sodium Pyruvate, Albumin,Transferrin, Myo-Inositol, Zink Sulphate and other Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, Ala-Glutamine, Glucose, Phenol Red, and Gentamycin
6 Vials for Oocyte Vitrification
6x2ml Prod. No. 2.22.010
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
6 Vials for Oocyte Warming
6x2ml Prod. No. 2.23.010
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
Stroebech Media is a new company providing IVF media for Assisted Reproduction Techniques in Animals.
We offer individual training and support as well as courses within our media and protocols, and extensive distributor training.
