50 ml Oil for overlay of media in glass bottle Prod. No. 6.09.050
Stroebech Oil is a pharmaceutical grade light mineral oil*), pre-washed and sterile filtered light paraffin oil. Oil is often the key to poor results, use only BEA tested and Peroxide Value (POV) tested oil as minimum standard quality control.
*) Liquid paraffin oil is a mineral oil and is a by-product of crude oil distillation.
Oil viscosity influences temperature, pH and osmolarity, with higher viscosity demonstrating higher yields after overlay in embryo culture in terms of blastocyst formation rates and cell counts.
Advantages: easier to handle, droplets are maintained better, and oil doesn’t spill over as easy.
Temperature, pH is better maintained and increased media osmolarity is avoided both during handling and during culture. This has a positive impact on blastocyst rate formation – especially for the longer culture periods required in some species.
The unit of viscosity is newton-second per square metre, which is usually expressed as pascal-second in SI units, cP.
We have increased the viscosity from < 30 cP to 95-140 cP.
Quality Control: we still apply the strict quality control tests of each batch which besides a bovine embryo assay (BEA) test also includes *) POV, sterility, and endotoxin tests.
*) The peroxide value (POV) is defined as the reactive oxygen contents expressed in terms of milliequivalents (meq) of free iodine per kilogramme of fat. It is determined by titrating iodine liberated from potassium iodide with sodium thiosulphate solution. Oils with POV well below 10 meq/kg are considered fresh – The limit for our oil is <0.1meq/kg