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Every media batch is provided with a Certificate of Analysis with a high level of Quality Control (QC) release parameters.
The most important is the Bovine Embryo Assay BEA Test. There are huge differences in reproductive physiology between the mouse and cow, therefore a MEA test is not sufficient QC for a bovine medium.
See more about our QC and Manufacturing here
As a customer you get a detailed Manual of Procedure and access to online support. Furthermore, there will be online training courses and individual troubleshooting sessions.
There is a detailed ICSI protocol available, including preparation of Semen, the ICSI procedure, culture conditions and vitrification.
IMPORTANT:
IVM, H-IVM, Holding Medium and the IVC Medium must be supplemented with 5 % Serum.
Oocyte Holding must be supplemented with 10 % -20 % Serum. The media also contain a synthetic serum replacement.
We recommend Fetal Bovine Serum from Sigma-Aldrich F2442. Note it is already sterile filtered, so no need to filter again. 5% serum corresponds to 1 ml to the 20 ml bottles
Contact us for custom made solutions.
500mlProd. No. 4.01.500
For retrieval of oocytes from Ovum Pick Up and for Embryo Flushing (ET)
Calcium Chloride, Glucose, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Heparin, Sodium Lactate, Sodium Pyruvate, HEPES and Gentamycin
20mlProd. No. 3.03.020
For in vitro maturation of oocytes in the laboratory
FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), Sodium Pyruvate, Albumin, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
5×1mlProd. No. 4.02.005
For denudation of mature equine oocytes
Pharmaceutical grade hyaluronidase, Sodium lactate, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium chloride dihydrate, Sodium Dihydrogen Phosphate Dihydrate, Magnesium Sulphate, Sodium Pyruvate, Albumin
50mlProd. No. 3.05.050
For washing of semen during centrifugation prior to ICSI
Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin
20mlProd. No. 3.07.020
For first step in vitro culture of embryos low glucose
Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Albumin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
20mlProd. No. 3.08.020
For in vitro culture of embryos from day 5 high glucose
Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, L-Carnitine, Ethanolamine, Sodium Pyruvate, Albumin, Transferrin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
3x2mlProd. No. 4.20.006
3 Vials for Embryo Vitrification
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts. The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
4x2mlProd. No. 4.21.008
4 Vials for Warming of Vitrified Embryos
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
6 Vials for Oocyte Vitrification
6x2ml Prod. No. 2.22.010
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
6 Vials for Oocyte Warming
6x2ml Prod. No. 2.23.010
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
Stroebech Media is a new company providing IVF media for Assisted Reproduction Techniques in Animals.
We offer individual training and support as well as courses within our media and protocols, and extensive distributor training.
