Equine Media Products

Every media batch is provided with a Certificate of Analysis with a high level of Quality Control (QC) release parameters.

The most important is the Bovine Embryo Assay BEA Test. There are huge differences in reproductive physiology between the mouse and cow, therefore a MEA test is not sufficient QC for a bovine medium.

See more about our QC and Manufacturing here

As a customer you get a detailed Manual of Procedure and access to online support. Furthermore, there will be online training courses and individual troubleshooting sessions.

There is a detailed ICSI protocol available, including preparation of Semen, the ICSI procedure, culture conditions and vitrification.

IMPORTANT:
IVM, Holding Medium and the IVC Medium must be supplemented with 5 % Serum.

Oocyte Holding must be supplemented with 10 % -20 % Serum. The media also contain a synthetic serum replacement.

We recommend Fetal Bovine Serum from Sigma-Aldrich F2442. Note it is already sterile filtered, so no need to filter again. 5% serum corresponds to 1 ml to the 20 ml bottles

Contact us for custom made solutions.

Equine OPU Medium

500mlProd. No. 4.01.500

For retrieval of oocytes from Ovum Pick Up and for Embryo Flushing (ET)

Key Ingredients:

Calcium Chloride, Glucose, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Heparin, Sodium Lactate, Sodium Pyruvate, HEPES and Gentamycin

Equine Oocyte Holding Medium

20mlProd. No. 3.12.020

For holding of Equine oocytes prior to maturation.

Key Ingredients:

Inorganic Salts, essential and non-essential Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Albumin, Glucose, Phenol Red, HEPES and Gentamycin

Equine IVM Medium

20mlProd. No. 3.03.020

For in vitro maturation of oocytes in the laboratory

Key Ingredients:

FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), Sodium Pyruvate, Albumin, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

Equine Sperm Pre Incubation Medium

20mlProd. No. 3.14.020

For pre-incubation of sperm, prior to IVF

Key Ingredients:

Essential and non-essential amino acids, PHE (Penicillamine, Hypotaurine, Epinephrine), Heparin, Albumin, Sodium Pyruvate, Inorganic Salts, Essential and Non-Essential Amino Acids, Ca-L-Lactate, Sodium Bicarbonate, ALA-Glutamine, and Gentamycin

Equine IVF Medium

20ml Prod. No. 3.15.020

For IVF of equine mature oocytes

Key Ingredients:

Essential and non-essential amino acids, PHE (Penicillamine, Hypotaurine, Epinephrine), Heparin, Albumin, Sodium Pyruvate, Inorganic Salts, Essential and Non-Essential Amino Acids, Ca-L-Lactate, Sodium Bicarbonate, ALA-Glutamine, and Gentamycin

Equine Hyaluronidase

5×1mlProd. No. 4.02.005

For denudation of mature equine oocytes

Key Ingredients:

Pharmaceutical grade hyaluronidase, Sodium lactate, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium chloride dihydrate, Sodium Dihydrogen Phosphate Dihydrate, Magnesium Sulphate, Sodium Pyruvate, Albumin

Equine Semen Wash Medium

50mlProd. No. 3.05.050

For washing of semen during centrifugation prior to ICSI

Key Ingredients:

Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin

Sperm Gradient Upper Layer 45%

20mlProd. No. 2.10.020
Key Ingredients:

Sodium bicarbonate, Silane-coated, colloid silica particles, Serum Albumin, Hepes, Sodium lactate, Sodium pyruvate, Sodium phosphate,Magnesium sulphate, Sodium chloride, Potassium chloride, Dextrose/Glucose, Calcium chloride

Sperm Gradient Lower Layer 90%

20mlProd. No. 2.11.020
Key Ingredients:

Sodium bicarbonate, Silane-coated, colloid silica particles, Serum Albumin, Hepes, Sodium lactate, Sodium pyruvate, Sodium phosphate,Magnesium sulphate, Sodium chloride, Potassium chloride, Dextrose/Glucose, Calcium chloride

Equine PVP Medium

1mlProd. No. 4.10.005

For decreasing the motility of sperm for ICSI

Key Ingredients:

PVP, Sodium Lactate, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium chloride dihydrate, Sodium Dihydrogen Phosphate Dihydrate, Magnesium Sulphate, Sodium Pyruvate, Albumin

Equine Swim Up Medium

50mlProd. No. 3.11.050

For sperm swim up

Key Ingredients:

Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Albumin, Inorganic Salts, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

Equine ICSI Medium

20mlProd. No. 3.06.020

For intra cytoplasmic injection of matured oocytes

Key Ingredients:

Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin, Albumin

Equine One Step IVC Medium w box

Equine One Step IVC Medium

20 ml Prod. No. 3.13.020

For in vitro culture of equine embryos from day 0 until blastocyst stage

Key Ingredients:

Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Albumin, Inorganic Salts, essential and non-essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

Equine IVC Medium 2 - Blastocyst

20mlProd. No. 3.08.020

For in vitro culture of embryos from day 5 high glucose

Key Ingredients:

Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, L-Carnitine, Ethanolamine, Sodium Pyruvate, Albumin, Transferrin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

Equine IVC Medium 1 - Cleavage

20mlProd. No. 3.07.020

For first step in vitro culture of embryos low glucose

Key Ingredients:

Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Albumin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

Equine Holding Medium

20mlProd. No. 3.09.020

For transfer and handling of embryos outside the incubator

Key Ingredients:

Inorganic Salts, essential and non-essential Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

Equine Stroebech Heavy Oil

50mlProd. No. 4.09.050

Peroxide tested pre-washed oil

Stroebech Heavy Oil is a pharmaceutical grade light mineral oil*), pre-washed and sterile filtered light paraffin oil.

Equine Vitrification Kit

3x2mlProd. No. 4.20.006

3 Vials for Embryo Vitrification

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts. The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos

Equine Warming Kit

4x2mlProd. No. 4.21.008

4 Vials for Warming of Vitrified Embryos

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos

Oocyte Vitrification Kit

6 Vials for Oocyte Vitrification 

6x2ml Prod. No. 2.22.010

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos

Oocyte Warming Kit

6 Vials for Oocyte Warming

6x2ml Prod. No. 2.23.010

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos

Contact us

Stroebech Media

Stroebech Media is a new company providing IVF media for Assisted Reproduction Techniques in Animals.

We offer individual training and support as well as courses within our media and protocols, and extensive distributor training.

See our Product brochure here

Contact Information

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