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Every media batch is provided with a Certificate of Analysis with a high level of Quality Control (QC) release parameters.
The most important is the Bovine Embryo Assay BEA Test. There are huge differences in reproductive physiology between the mouse and cow, therefore a MEA test is not sufficient QC for a bovine medium.
See more about our QC and Manufacturing here
As a customer you get a detailed Manual of Procedure and access to online support. Furthermore, there will be online training courses and individual troubleshooting sessions (also online- until we can travel again).
The maturation and culture media contain serum and are ready-to-use and require no supplements.
The media range applies to in vitro production of bovine embryos from both Ovum Pick Up and Slaughterhouse ovary collection. The media can also be used to produce sheep, goat, buffaloes and camelid embryos. For endangered species, ZOO- and wildlife animals
Contact us for custom made solutions.
For retrieval of oocytes from Ovum Pick Up and for Embryo Flushing (ET)
Calcium Chloride, Glucose, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Heparin, Sodium Lactate, Sodium Pyruvate, HEPES and Gentamycin
For handling of oocytes and embryos outside of the incubator
Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Inorganic Salts, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin
For in vitro maturation of oocytes in the laboratory
Serum, FSH and LH, BSA, Epidermal Growth Factor (EGF), Midkine (MK), Sodium Pyruvate, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
For denudation of mature equine oocytes
Bovine Pharmaceutical grade hyaluronidase, Serum Albumin, Sodium lactate, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium chloride dihydrate, Sodium Dihydrogen Phosphate Dihydrate, Magnesium Sulphate, Sodium Pyruvate
For first step in vitro culture of embryos low glucose
Serum, Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, BSA, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
For in vitro culture of embryos from day 5 high glucose
Serum, Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, BSA, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin
3 Vials for Embryo Vitrification
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts. The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
4 Vials for Warming of Vitrified Embryos
Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos
Stroebech Media is a new company providing IVF media for Assisted Reproduction Techniques in Animals.
We offer individual training and support as well as courses within our media and protocols, and extensive distributor training.
