Equine Media Products

Every media batch is provided with a Certificate of Analysis with a high level of Quality Control (QC) release parameters.

The most important is the Bovine Embryo Assay BEA Test. There are huge differences in reproductive physiology between the mouse and cow, therefore a MEA test is not sufficient QC for a bovine medium.

See more about our QC and Manufacturing here

As a customer you get a detailed Manual of Procedure and access to online support. Furthermore, there will be online training courses and individual troubleshooting sessions (also online- until we can travel again).

The maturation and culture media contain serum and are ready-to-use and require no supplements.

The media range applies to in vitro production of bovine embryos from both Ovum Pick Up and Slaughterhouse ovary collection. The media can also be used to produce sheep, goat, buffaloes and camelid embryos. For endangered species, ZOO- and wildlife animals

Contact us for custom made solutions. 

For retrieval of oocytes from Ovum Pick Up and for Embryo Flushing (ET)

Key Ingredients:

Calcium Chloride, Glucose, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Heparin, Sodium Lactate, Sodium Pyruvate, HEPES and Gentamycin

For handling of oocytes and embryos outside of the incubator

Key Ingredients:

Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Inorganic Salts, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

For in vitro maturation of oocytes in the laboratory

Key Ingredients:

Serum, FSH and LH, BSA, Epidermal Growth Factor (EGF), Midkine (MK), Sodium Pyruvate, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

For denudation of mature equine oocytes

Key Ingredients:

Bovine Pharmaceutical grade hyaluronidase, Serum Albumin, Sodium lactate, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium chloride dihydrate, Sodium Dihydrogen Phosphate Dihydrate, Magnesium Sulphate, Sodium Pyruvate

Equine Semen Wash Medium

For washing of semen during centrifugation prior to ICSI

Key Ingredients:

Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin

Equine Swim Up Medium

For sperm swim up

Key Ingredients:

Serum Albumin, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Inorganic Salts, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

Equine PVP Medium

For slowing down sperm for ICSI

Key Ingredients:

PVP, Serum Albumin, Sodium lactate, Glucose, Sodium Hydrogen Carbonate, Potassium Chloride, Calcium chloride dihydrate, Sodium Dihydrogen Phosphate Dihydrate, Magnesium Sulphate, Sodium Pyruvate

Equine ICSI Medium

For intra cytoplasmic injection of matured oocytes

Key Ingredients:

Serum Albumin, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

Equine IVC Medium 1 - Cleavage

For first step in vitro culture of embryos low glucose

Key Ingredients:

Serum, Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, BSA, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

Equine IVC Medium 2 - Blastocyst

For in vitro culture of embryos from day 5 high glucose

Key Ingredients:

Serum, Insulin, Hyaluronic Acid, Calcium L-Lactate, Selen, BSA, L-Carnitine, Ethanolamine, Sodium Pyruvate, Transferrin, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin

Equine Transfer Medium

For Transfer of equine in vitro or in vivo produced embryos

Key Ingredients:

BSA, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

Oocyte Holding Medium

For holding of oocytes prior to maturation 

Key Ingredients:

BSA, Inorganic Salts, Essential and Non-Essential Amino Acids, Vitamins, Sodium Bicarbonate, Alanyl Glutamine, Glucose, Phenol Red, Tween 80, HEPES and Gentamycin

Equine Stroebech Heavy Oil

50 ml Peroxide tested Pre-washed Oil

Stroebech Oil is a pharmaceutical grade light mineral oil*), pre-washed and sterile filtered light paraffin oil.

Equine Vitrification Kit

3 Vials for Embryo Vitrification

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts. The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos

Equine Warming Kit

4 Vials for Warming of Vitrified Embryos

Key Ingredients:

Sucrose, Ficoll, Dimethyl Sulphoxide (DMSO) Ethylene Glycol, Glucose, Sodium Pyruvate, Albumin, Inorganic Salts The cryoprotective ingredients work by dehydrating embryos combined with an influx of high concentrations of cryoprotectants by increasing osmolality stepwise from 290 mOsm/kg to 865 mOsm/kg and the reverse procedure for warming (rehydration of embryo and removal of cryoprotectants) Above steps are essential for rapid cooling into LN2, which takes place from 30 °C to −196 °C in less than one second See the detailed protocol on Vitrification and Warming of embryos