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Semen Wash Medium

For washing of semen during centrifugation prior to in vitro fertilization

Description and Prod. No.

50 ml non-capacitating medium in glass bottle for semen washing, Prod. No. 5.05.050

The medium contains a very low concentration of Sodium Bicarbonate and is phosphate buffered and must therefore not be CO
2 equilibrated

The medium is ready-to-use and does not need any supplements Medium should be preheated to 38.8°C prior to use with the lid on

Key Ingredients:

Calcium Chloride, Magnesium Sulphate, Potassium Chloride, Sodium Bicarbonate, Sodium Chloride, Sodium Dihydrogen Phosphate, Sodium Lactate, Sodium Pyruvate, Phenol Red and Gentamycin

Advantages of using a non-gradient and non-capacitating medium

  • The capacitation of the spermatozoa is delayed until they are in the actual IVF medium with the oocytes
  • Spermatozoa preserve energy for the subsequent capacitation, acrosome reaction and fertilization
  • Potential toxic gradients and excessive centrifugation is avoided
  • The Semen Wash Medium also applies very well to sex-sorted semen

Quality Control Tests and Manufacturing

  • Sterility tested (Fungal and Bacterial)
  • Osmolality tested and pH tested
  • Endotoxin tested
  • Manufacturer is ISO9001 and ISO13485 certified
  • Controlled environment and to human standards
  • Bovine Embryo Assay (BEA)
  • Batch analysis certificate available

Storage:

  • Store in original unopened container refrigerated 3-9 °C and protected from light
  • Do not expose to temperatures higher than 39°C
  • Do not freeze product it may precipitate < 2°C
  • The medium must be used within 10 days after opening, providing it has been
  • handled aseptically and is kept refrigerated after opening
  • Discard unused medium if it has been warmed

Contact: www.stroebech-media.com - Phone: +45 5353 8743