For the in vitro maturation of oocytes outside the incubator
20 ml HEPES IVM medium in glass bottle, Prod. No. 5.04.020
The medium is HEPES buffered and does not require CO
2 equilibration, but it can also be used in a CO
2 Incubator (recommended without vial lid, if oocytes arrive to the laboratory before 20 hours of after IVM)
The medium is ready-to-use and does not need any supplements
Medium should be preheated to 38.8°C prior to use
Leptin, HEPES, FSH and LH, Epidermal Growth Factor (EGF), Midkine (MK), L-Carnitine, Sodium Pyruvate, Albumin, Inorganic Salts, Essentiel and Non-Essentiel Amino Acids, Vitamins, Sodium Bicarbonate, L-Glutamine, Glucose, Phenol Red, and Gentamycin